This study presents a comprehensive and systematic examination of a revered Qurn manuscript, commonly attributed to the third Islamic Caliph, Uthmn b. Affn (d. 35/656), housed at the Topkap Palace Museum in Istanbul, Türkiye (identified as H.S. 32). Halaseh offers a meticulous analysis of the manuscript’s codicological, paleographic, and orthographic characteristics, explores its emendations, production date, and traces the manuscript’s journey first to Cairo and then to Istanbul. Additionally, the study examines and categorizes its textual variants, including what are considered today as non-canonical and some that are not previously attested in the major qirt literature. This work not only sheds light on the transmission of the Qurnic text but also establishes a comprehensive framework for researching Qurn manuscripts. By integrating methods for examining these manuscripts as both physical artifacts and scriptural texts, Halaseh presents a holistic methodological approach to their scholarly study.