This book provides a comprehensive overview of multimodal and combined techniques used for imaging applications in Chemical Biology, including afterglow luminescence imaging, chemiluminescence imaging, delayed fluorescence imaging, AIE fluorescence imaging, and many more techniques. As well as providing in-depth detail on the application of these techniques, the advantages and disadvantages of each are also discussed. Additionally, Imaging Tools for Chemical Biology also covers the use of chemical probes for tagging biomolecules and therapeutic applications. The current state of the art in biological imaging and future directions for the field are also discussed and evaluated. This book is a valuable resource and ideal for researchers new to the area, as well as experienced chemists interested in developing probes and biologists interested in utilising probes in their research.