This book serves as an extensive resource on electrophysiology, offering a detailed exploration of techniques used to study ion channels, synaptic function, and neural circuits. It provides in-depth descriptions of various patch-clamp recording methods, including single-channel, whole-cell, perforated patch, and in vivo approaches. In addition to patch-clamp techniques, the book covers intracellular sharp electrode recordings, extracellular electrophysiology, and high-density multi-electrode arrays, ensuring broad applicability across diverse experimental settings.
A key strength of this book is its focus on diverse cell types and models, including neurons, glial cells, immune cells, organoids, human iPSC-derived neurons, and intracellular organelles. It also explores advanced techniques such as robotic automation, two-photon targeted patch-clamp, and optogenetics-based circuit mapping, ensuring its relevance to contemporary neuroscience research. Designed for both novice and experienced researchers, the book provides guidance on fundamental equipment, experimental design, and troubleshooting. It serves as an essential resource for neuroscientists, electrophysiologists, and biomedical researchers investigating electrical activity at cellular and network levels.
Contents:
- Introduction:
- How to Patch a Cell (Hovy Ho-Wai Wong, Olivier Camiré & P Jesper Sjöström)
- Current Clamp, Voltage Clamp and Dynamic Clamp (Jean-Marc Goaillard, Ede Rancz & Nicolas Wanaverbecq)
- Patch-Clamp Recordings from Channels and Cultures:
- Single-Channel Recordings from Planar Lipid Bilayers (Nils Drexler & Indra Schroeder)
- Single-Channel Recordings (Jelena Baranovic & Andrew Plested)
- Whole-Cell Patch-Clamp Recordings in Cultured Neuronal Cells (Paul Kammermeier)
- Whole-Cell Patch-Clamp Recordings in Organotypic Slices (Mathieu Letellier & Lorenzo A Cingolani)
- Whole-Cell Patch-Clamp Recordings in 3D Organotypic Spinal Cultures (Rossana Rauti and Laura Ballerini)
- Whole-Cell Patch-Clamp Recordings from Acute Slices:
- Quantifying Synaptic Properties (Nicole F Wong and Matthew A Xu-Friedman)
- Channelrhodopsin-2-Assisted Circuit Mapping and Its Applications (Leopoldo Petreanu)
- Multiple Whole-Cell Recordings (Hovy Ho-Wai Wong, Aurore Thomazeau & P Jesper Sjöström)...